Identification of differentially expressed genes in human lung

Identification of differentially expressed genes in human lung

Identification of differentially expressed genes in human lung adenocarcinoma: ERGIC3 as a novel lung cancer-related gene Mingsong Wu Department of Cell Biology and Genetics Zunyi Medical University China Las Vegas 2014.10

Part I 1 Background 1 epidemiology of lung cancer ortality in lung cancer in selected count 1950 2006 Jemal et al.Cancer Epidemiology, Biomarkers & Prevention. (2010)

Subtype of lung cancer lung cancer small-cell small-cell lung lung carcinoma carcinoma

non-small non-small cell cell lung lung carcinoma carcinoma squamous cell carcinoma large-cell carcinoma

adenocarcinoma New tumor marker of lung cancer for early diagnosis and treatment To investigate genetic mechanism and new target 1.2 Molecular basis

of LC 1.3 Suppression subtractive hybridization(SSH) cDNA Microarray mRNA Differential display DD Representational difference analysis RDA Suppression subtractive hybridization, SSH

2 Results Construction of AC-associated li 2.1.1 Total RNA & mRNA total RNA mRNA

2.1.2 Analysis of Rsa I digestion digestion of double cDNA 2.1.3 Ligation efficiency analysis 35 2.1.4 Supression PCR

2.2 Clone of EST 2nd PCR Product pGEM T-easy Vector White clones PCR Digestion

Sequence FSL 485 upregulated RSL 172 down- Analysis 2.3 Bioinformatic analysis

2.3.1 BLAST Gene 177 new 152 Unknown EST: 44 Gene 59 new 54 Unknown EST: 10 2.3.2 GO Ontology

2.3.3 Biocatar pathway RSL 2.3.4 Biocatar pathway FSL 2.4 Sreen of SSHlibraries

Higher quality Higher efficiency Part II 1 Background (Endoplasmic reticulum-Golgi intermediate compartment 3 ERGIC3) Cycles between the endoplasmic reticulum and Golgi II type transmembrane protein, 383 aa, 43.2 kDa Herzog et al. 2006

Mutation: COPII 25 Accumulation of protein and lipid in ER Inhibits ER stress-induced cell death FUNCTION? TUMOR MARKER 2 RESULTS Expression of ERGIC3(2^CT)

2.1 Expression of ERGIC3 in NSCLC 2.1.1 mRNA of ERGIC3 0.025 P=0.0001

0.020 0.015 0.010 0.005 0.000 Tu m

or n m n o ig al

nt a n 2.1.2 Protein of ERGIC3 2.1.3 Protein of ERGIC3 Evaluation of immunohistochemical positivity in lung cancer tissues

Tumour and clinicopathological features Gender Male Female Histological type* AC SCC

TNM stage I + II III + IV Differentiation* Poor Well or Moderate Smoking No Yes

Score + ++ +++ 3/17(17.6)

1/18(5.6) 5/17(29.4) 4/18(22.2) 4/17(33.5) 5/18(27.8) 5/17(29.4) 8/18(44.4)

0 4/13(30.8) 6/22(27.3) 3/13(23.1) 7/22(31.8) 2/13(15.4)

9/22(40.9) 4/13(30.8) 3/20(15.0) 1/15(6.7) 5/20(25.0) 4/15(26.7) 7/20(35.0)

2/15(13.3) 5/20(25.0) 8/15(53.3) 4/11(36.4) * 0 2/11(18.2) * 7/24(29.2) *

1/11(9.1) * 8/24(33.3) * 4/11(36.4) * 9/24(37.5) * 1/17(5.9) 3/18(16.7)

4/17(23.5) 5/18(27.8) 4/17(23.5) 5/18(27.8) 8/17(47.1) 5/18(27.8) Positive

rate:88.57% Score: , no staining;AC>SCC +, <30%; ++, 3160%; +++, >60% positive cells 2.1.4 Location of ERGIC3 2.1.4 Location of ERGIC3 2.2 Functions of ERGIC3 in lung

cancer 2.2.1 ERGIC3 promotes cellular growth 2.2.2 ERGIC3 promotes cellular migration 2.2.3 Stable overexpression of ERGIC3 promotes

cellular migration 3 Summ ary

Library of differentially expressed genes in lung cancer. Potential targets for diagnostic & therapeutic usage. Two lung cancer associated genes. ERGIC3 may be a new biomarker for lung cancer. ERGIC3 may be a novel candidate oncogen for lung cancer.

Recently Viewed Presentations

  • Aucun titre de diapositive

    Aucun titre de diapositive

    Karina. Mandre. Page . ... LNH 87 PERIPHERAL T CELL LYMPHOMA Cox's analysis survival OVERALL SURVIVAL OF THE 156 PATIENTS WITH ANGIOIMMUNOBLASTIC T-CELL LYMPHOMAS: LNH 87-LNH93 Diapositive 7 Diapositive 8 Event Free Survival Diapositive 10 WHERE IS THE PROBLEM ?...
  • CSE 142 Python Slides - Building Java Programs

    CSE 142 Python Slides - Building Java Programs

    public static boolean bothOdd(int n1, int n2) { return (n1 % 2 != 0 && n2 % 2 != 0); } "Short-circuit" evaluation Java stops evaluating a test if it knows the answer. && stops early if any part of...
  • The Network Layer

    The Network Layer

    Network Layer IPv6 Slides were original prepared by Dr. Tatsuya Suda Contents 6. IPv6 2. IPv6 IPv4 (the standard IP protocol) is limited IP is running out of ...
  • Femme en situation de handicap

    Femme en situation de handicap

    Chiffres 1 femme sur 5 au Canada est handicapée 55% des personnes handicapées sont des femmes 60% des femmes en situation de handicap vivront de la violence au cours de leur vie adulte. Violence conjugale: femmes en situation de handicap...
  • Foundations in Microbiology Sixth Edition

    Foundations in Microbiology Sixth Edition

    Arial Times New Roman Arial Unicode MS Default Design Foundations in Microbiology Sixth Edition Survey of Microbial Disease Phenotypic Methods Genotypic Methods Immunological Methods Specimen Collection and Laboratory Methods Slide 7 Slide 8 Phenotypic Methods Slide 10 Slide 11 Genotypic...
  • Demystifying Digital Scholarship 1 - McMaster University

    Demystifying Digital Scholarship 1 - McMaster University

    Twitter. Twitter is an entirely different animal than Facebook in terms of what information is available and how it's organized, but the way you gain access to collect it is much the same.
  • Ramayana Performances - ASAN 310: South &amp; Southeast Asia

    Ramayana Performances - ASAN 310: South & Southeast Asia

    Arial MS Pゴシック Calibri Office Theme Ramayana in Performance Sept. 15, 2011 Slide 2 Ramayana Art Ramayana Art from the Metropolitan Museum, NYC Story Cloths Ramayana Performances - India Ramayana Performances Southeast Asia Ramayana Performance Indonesia (Java/Bali) Ramayana Puppetry -...
  • Phase 3 Provider Summits Transforming the Health of

    Phase 3 Provider Summits Transforming the Health of

    We are a leader in healthcare with a comprehensive portfolio of innovative solutions which includes coverage for Medicaid, Medicare and the Health Insurance Marketplace. We also contract with other healthcare and commercial organizations to provide specialty services to help people...